wizard kit Search Results


ivis spectrum system  (Revvity)
96
Revvity ivis spectrum system
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Ivis Spectrum System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ivis spectrum system - by Bioz Stars, 2026-06
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opera phenix high content screening system  (Revvity)
96
Revvity opera phenix high content screening system
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Opera Phenix High Content Screening System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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opera phenix high content screening system - by Bioz Stars, 2026-06
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chemdraw professional 20 0 program  (Revvity)
94
Revvity chemdraw professional 20 0 program
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Chemdraw Professional 20 0 Program, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phenovue cell painting jump kit  (Revvity)
94
Revvity phenovue cell painting jump kit
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Phenovue Cell Painting Jump Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phenovue cell painting jump kit - by Bioz Stars, 2026-06
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janus liquid handling system  (Revvity)
94
Revvity janus liquid handling system
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Janus Liquid Handling System, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard genomic purification dna kit  (Promega)
90
Promega wizard genomic purification dna kit
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Wizard Genomic Purification Dna Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard hmw dna extraction kit  (Promega)
90
Promega wizard hmw dna extraction kit
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Wizard Hmw Dna Extraction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard genomic purification kit  (Promega)
90
Promega wizard genomic purification kit
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Wizard Genomic Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard genomic kit  (Promega)
90
Promega wizard genomic kit
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Wizard Genomic Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna purification kit  (Promega)
90
Promega dna purification kit
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard prep kit  (Promega)
90
Promega wizard prep kit
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Wizard Prep Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wizard dna extraction kit  (Promega)
90
Promega wizard dna extraction kit
THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system <t>(IVIS)</t> images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.
Wizard Dna Extraction Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system (IVIS) images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.

Journal: Frontiers in Immunology

Article Title: Thrombospondin-1 mitigates osteoarthritis progression by inhibiting mechanical stress-induced chondrocyte ferroptosis via the integrin/YAP pathway

doi: 10.3389/fimmu.2025.1577234

Figure Lengend Snippet: THBS1 protects articular cartilage and delays the progression of osteoarthritis. (A) Flowchart of animal experiment. (B) In vivo imaging system (IVIS) images of 10-week-old ray that were delivered with AAV-GFP, AAV-THBS1 or PBS. (C) Real-time PCR of THBS1 in articular cartilage of the AAV-GFP group and AAV-THBS1 group. (D) Representative immunofluorescence images of THBS1 in articular cartilage of the indicated groups. Scale bars, 50 μm. (E) Quantification of immunofluorescence analysis (n=3 for each group). (F) Representative images of Micro-CT of the AAV-GFP group, AAV-THBS1 group or CON group (n=6). Arrows show the formation of osteophytes. (G) Osteophyte number assay based on Micro-CT (n=6 for each group). (H) Representative images of safranin O staining of the AAV-GFP group, AAV-THBS1 group or CON group. Scale bars, 200 μm (low field), 100 μm (high field). (I) Osteoarthritis Research Society International (OARSI) score of OA based on the results of safranin O staining (n=6 for each group). (J) Immunohistochemical assay of Aggrecan, Col2, ADAMTS-5 and MMP-9 in articular cartilage of the indicated groups. Scale bars 200 μm. (K-N) Quantification of immunohistochemical analysis (n=3 for each group). Data were presented as the mean ± SD. *P<0.05, **P<0.01.

Article Snippet: Fourteen days post-surgery, in vivo 3D optical imaging was performed using the IVIS Spectrum system (PerkinElmer, USA) to assess transfection efficiency.

Techniques: In Vivo Imaging, Real-time Polymerase Chain Reaction, Immunofluorescence, Micro-CT, Staining, Immunohistochemical staining